| 质粒名称: | SV40 1: pBSSVD2005 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21826 |
| 存储实验室: | David Ron |
|
载体骨架基本信息 |
|
| 载体名称: | Bluescribe |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3200 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SV40 Large T,Simian virus-40 large-T antigen |
| 物种来源: | 猿猴病毒 |
| 插入大小: | 2800 bp |
| 基因突变: | likely expresses aa109-708 of the large T antigen |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T3 |
| 3' 测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | SV40 1: pBSSVD2005 was a gift from David Ron (Addgene plasmid # 21826 ; http://n2t.net/addgene:21826 ; RRID:Addgene_21826) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS SV40 1: pBSSVD2005 5984 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS SV40 1: pBSSVD2005
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5984)
TITLE SV40 large T antigen for immortalizing cells
REFERENCE 2 (bases 1 to 5984)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5984
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(11..28)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(262..281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1564..1583
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
primer_bind 1974..1992
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2030..2052)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2152..2171
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(2238..2693)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2375..2396)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2587..2606
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2819..2841
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2833..2850
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2834..2850
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2857..2876
/label=T7
/note="T7 promoter, forward primer"
promoter 2857..2875
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 2921..3055
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2945..2964)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2999..3018
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(4809..4829)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 4959..5024
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
promoter complement(5314..5671)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5328..5463
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5382..5401)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 5628..5648
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter complement(5725..5743)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5764..5780)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5764..5780)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5777..5799)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5788..5804
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5812..5842)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5857..5878
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
1981 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
2041 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
2101 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
2161 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
2221 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
2281 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
2341 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
2401 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
2461 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
2521 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
2581 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
2641 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
2701 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
2761 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
2821 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
2881 ttctcatgtt tgacagctta tcatcgataa gcttcagctg gatccagaca tgataagata
2941 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
3001 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
3061 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
3121 caagtaaaac ctctacaaat gtggtatggc tgattatgat catgaacaga ctgtgaggac
3181 tgaggggcct gaaatgagcc ttgggactgt gaatcaatgc ctgtttcatg ccctgagtct
3241 tccatgttct tctccccacc atcttcattt ttatcagcat tttcctggct gtcttcatca
3301 tcatcatcac tgtttcttag ccaatctaaa actccaattc ccatagccac attaaacttc
3361 attttttgat acactgacaa actaaactct ttgtccaatc tctctttcca ctccacaatt
3421 ctgctctgaa tactttgagc aaactcagcc acaggtctgt accaaattaa cataagaagc
3481 aaagcaatgc cactttgaat tattctcttt tctaacaaaa actcactgcg ttccaggcaa
3541 tgctttaaat aatctttggg cctaaaatct atttgtttta caaatctggc ctgcagtgtt
3601 ttaggcacac tgtactcatt catggtgact attccagggg gaaatatttg agttctttta
3661 tttaggtgtt tcttttctaa gtttacctta acactgccat ccaaataatc ccttaaattg
3721 tccaggttat taattccctg acctgaaggc aaatctctgg actcccctcc agtgcccttt
3781 acatcctcaa aaactactaa aaactggtca atagctactc ctagctcaaa gttcagcctg
3841 tccaagggca aattaacatt taaagctttc cccccacata attcaagcaa agcagctgct
3901 aatgtagttt taccactatc aattggtcct ttaaacagcc agtatctttt tttaggaatg
3961 ttgtacacca tgcattttaa aaagtcatac accactgaat ccattttggg caacaaacag
4021 tgtagccaag caactccagc catccattct tctatgtcag cagagcctgt agaaccaaac
4081 attatatcca tcctatccaa aagatcatta aatctgtttg ttaacatttg ttctctagtt
4141 aattgtaggc tatcaacccg ctttttagct aaaacagtat caacagcctg ttggcatatg
4201 gttttttggt ttttgctgtc agcaaatata gcagcatttg cataatgctt ttcatggtac
4261 ttatagtggc tgggctgttc ttttttaata cattttaaac acatttcaaa actgtactga
4321 aattccaagt acatcccaag caataacaac acatcatcac attttgtttc cattgcatac
4381 tctgttacaa gcttccagga cacttgttta gtttcctctg cttcttctgg attaaaatca
4441 tgctccttta acccacctgg caaactttcc tcaataacag aaaatggatc tctagtcaag
4501 gcactataca tcaaatattc cttattaacc cctttacaaa ttaaaaagct aaaggtacac
4561 aatttttgag catagttatt aatagcagac actctatgcc tgtgtggagt aagaaaaaac
4621 agtatgttat gattataact gttatgccta cttataaagg ttacagaata tttttccata
4681 attttcttgt atagcagtgc agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga
4741 gttctattac taaacacagc atgactcaaa aaacttagca attctgaagg aaagtccttg
4801 gggtcttcta cctttctctt cttttttgga ggagtagaat gttgagagtc cgcggtagcc
4861 tcatcatcac tcgagggcat ttcttctgag caaaacaggt tttcctcatt aaacgcgttc
4921 caccactgct cccattcatc agttccatag gttggaatct aaaatacaca aacaattaga
4981 atcagtagtt taacacatta tacacttaaa aattttatat ttaccttacc aggatttaag
5041 gaagaagcaa atacctcagt tgcatcccag aagcctccaa agtcaggttg atgagcatat
5101 tttactccat cttccatttt cttgtacaga gtattcattt tcttcatttt ttcttcatct
5161 cctcctttat caggatgaaa ctccttgcat ttttttaaat atgcctttct catcagagga
5221 atattccccc aggcactcct ttcaagacct agaaggtcca ttagctgcaa agattcctct
5281 ctgtttaaaa ctttatccat ctttgcaaag ctttttgcaa aagcctaggc ctccaaaaaa
5341 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
5401 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
5461 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
5521 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
5581 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
5641 cacattccac agctggttct ttccgcctca gaaggtaccc ggggatcctc tagagtcgac
5701 ctgcaggcat gcaagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
5761 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
5821 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
5881 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
5941 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgct
//
